First identification of NDM-1 Metallo ß –Lactamase among clinical isolates of Klebsiella pneumonia isolates in Hilla hospitals, Iraq

Fatima Moeen Abbas; Eman Mohammad Jarallah

Authors

Fatima Moeen Abbas College of science for women, Babylon university Author
Eman Mohammad Jarallah College of science, Babylon university, Iraq. Author

Keywords:

Klebsiella pneumonia,, NDM-1 Metallo ß –Lactamase,, Clinical isolates,, PCR.

Abstract

This study analyzed the prevalence of blaNDM-1 in clinical isolates of Klebsiella pneumoniae. During the period from
April to August 2011, a total of 801 various clinical samples were collected from different hospitals in Hilla city. Of
these,117 isolates were specified as K.pneumoniae . High prevalence of K.pneumoniae isolates were detected in
stool samples 38 (27%) followed by sputum 19 (15%). All 117 K.pneumoniae isolates were primarily screened for 
- lactams resistance, 91 (78%) were found to be screen positive. β- lactam resistance isolates were underwent
antimicrobial susceptibility toward 26 antibiotics by Kirby-Bauer disk diffusion methods .Higher resistance rate was
recorded for penicillins (carbenicillin and ampicillin) with rates of resistance of (99%) and (94.5%),respectively
.Carbapenem resistance was reported in 17 (18.7%) K. pneumoniae isolates. Phenotypic detection of metallo β-
lactamase by imipenem-EDTA disk identified a proportion of 65% as metallo β-lactamase producers. The presence
of blaNDM-1 gene was checked by Polymerase Chain reaction (PCR) and confirmed in 3(17.6%) of isolates.