PER β- lactamase in Klebsiella pneumoniae resistant to carbapenem from Al-Hillah Teaching Hospital environment

Abstract

The aim of this  work is to identify the existence of K.pneumoniae recovered  from various places of Al-Hillah Teaching Hospital environment / Hillah city/ Babylon Province and detect bla_PER gene among carbapenem resistant isolates. One hundred samples were collected by swabbing various environmental locations between the period from October, 2017 and January, 2018.Klebsiella pneumoniae were detected in 13(13%) location. ESBL production was screened phenotypically using CHROMagar technique. Ten (77%) K.pneumoniae isolates with positive result. Disk diffusion method was used to test susceptibility of ESBL-K.pneumoniae producers .Higher prevalence rates of resistance were recorded for ampicillin (80%)  and amoxicillin-clavulanic acid (70%)  antibiotics. Two isolates were resistant to meropenem, these were checked for the existence of PERβ-lactamase using Polymerase Chain Reaction (PCR) technique. bla_PER gene was detected in 1 (50%) selected isolate.