Comparison between Newcastle disease vaccine and liposomal entrapped Newcastle disease vaccine in chicks
Abstract
The study aimed to evaluate the immune response of chicks against Newcastle disease vaccine between conventional and liposomal Newcastle vaccine, the experiment includes: 120, broiler chicks, one day- old, distrusted equally in to four groups, 30 birds in each; unilamellar, conventional and positive and negative control, the experiment was conducted to determine the ability of unilamellar liposome-encapsulated ND vaccine to protected chicks from field infection comparison with conventional vaccine, the antibody titer against ND vaccine was enhanced vaccination titer of birds at 10 days post the first immunization of conventional ND 639.00±4.82, and unilamellar liposome-encapsulated ND vaccine 729.40±5.64 and the second immunization of conventional ND 720.80±1.49 and unilamellar liposome-encapsulated ND vaccine 1207.90±3.50. The IFN-Y levels in unilamellar liposome-encapsulated ND vaccinated group was higher level antibodies titer than conventional ND and displayed at day 10day post-vaccination 113.00 ±1.83 and at 20 day post-vaccination 151.80 ±1.25 as compared with positive conventional ND vaccine 101.60 ±1.66 and negative control 122.00± 2.05. Challenge test was achieved by virulent ND 107.5 ELD50 at 40 days old of vaccinated birds and control; post 20 days of second vaccination orally, the protection of vaccines liposome formulate share 90% safeness and become resisted bird to infection higher than conventional control 80% vaccinated bird that endorsed superior formula proper immune cells based on local mucosal immunity and cell mediated immunity were improved protection percent. The study has concluded achieved shorter in onset time protection started and long-lasting immunity against repeated infection in maximized the immunological titer of bird protection as compared with conventional vaccination process.
