Determination of vitamin C levels in callus, regenerated shoots and tap roots from carrot (Daucus carota L.)

Abstract

One of the essential root vegetables that is abundant in bioactive substances like vitamin C is the carrot. There is a great deal of concern and research about the variation in vitamin C content of fresh Daucus carota. With an emphasis on tissue culture especially, this study seeks to advance existing approaches in the field of plant biotechnology. callus induction utilizing MS media supplemented with various concentrations of NNA and BA from 2cm pieces of hypocotyls. through using MS media supplemented with 1 mg/L NAA + 4 mg/L TDZ under light luminescence, shoots were regenerated from calluses. Vitamin C levels were measured in the mother plant's tap roots, regenerated shoots, and callus tissue by using UV spectrophotometry method. The findings showed that vitamin C concentration in callus tissue and newly grown shoots was superior to that in tap roots and mother plants, and that the level of vitamin C increased in newly grown shoots of plants.