Pathological study of the main pulmonary infections isolated from sheep in Holy Kerbala province

Abstract

In this paper we updating some data  previously publishing at 2019, present work was designed to implement two goals ,the first one aimed to isolate the most important  causes (parasites and bacteria) of pulmonary infection in sheep. The second goal endeavored to experimental study the effect the main bacterial isolation in mice .The first goal achieved by examining  100 lungs at different area of Karbalaa for the period  from  November 2017 to  March 2018.Atotal of  52 lungs of sheep were examined macroscopically and histopathological  and  the main condemned  diseases in this study were reported  hydatidosis. The total number of bacterial isolates which identified by culturing and biochemical tests were 51 samples. They were examined grossly for pulmonary lesion, 2 pieces of lesion were collected in 10% natural  buffered formalin for fixation and sent to the laboratory for histopathological examination .The samples for bacteriological studies were taken directly from lungs aseptically after slaughter the animals and from each sample bacterial isolation were done on macConky agar ,blood agar and mannitol salt agar and biochemical tests .These bacterial samples were include: Klebsiella pneumonia 10 (19.6%), Staphylococcus aureus 9 (17.6%), Escherichia coli 8 (15.7%), Pasturella multocida 7 (13.7%), Streptococuss pneumonia 6 (11.8%),Escherichia coli O157 4 (7.8%), Corynebacterium psudotuberculosis 3 (5.9%), Proteusspp2 (3.9%)and Enterobactor spp 2 ( 3.9%). Pneumonic lesions were classified microscopically into four types according to the inflammatory exudates: 1-Chronic interstitial Pneumonia was found in 19 pneumonic infected (44.2%)and  characterized by thickening in alveolar wall  and interstitial tissue due to infiltration of inflammatory cells .Most of the  associated bacterial isolates were:6 isolate of Klebsialla pneumonia,5 isolate of Pasurella multocida and 4  isolate of Streptococus pneumonia and 4 isolate of Staphylococcus aureus. 2-Suppurative bronchopneumonia was found in 15 pneumonic infected (34.9%) and characterized by suppurative exudates in bronchioles and bronchi with infiltration of inflammatory cells composed mainly of neutrophils .Most of the associated bacterial isolates were :5 isolate of Staphylococcus auerus,4 isolate Klebsiella pneumonia, 2 isolate of Streptococcus pneumonia ,2 isolate of Proteus spp and 2 isolate of Escherichia coli O157. 3.fibrinous pleuropneumonia was found in 6 of pneumonic infected (13.9%) and characterized by fibrinous  exudates in pulmonary tissue  with cellular infiltration .Most of associated bacterial isolates  were :2 isolate of Escherichiacoli O157 ,2 isolate of Enterobactor spp and 2 isolate of Pasturella multocida. 4.Granulomatous pneumonia was found in 3 ( 7 %) of pneumonic infected and characterized by granulomatous inflammatory reaction which consisted of central caseous necrosis surrounded by inflammatory cell and surrounded by fibrous connective tissue. Most of associated bacterial isolates were :3 isolate of Corynabacterium psudotuberculosis. Conclusion shows that  examined lungs samples from different cities in Kerbala provelinc were  infected with  parasites and  bacterial causes which associated with histopatholigical lesion mainly  granulomatious and fibrinouse and suppurative  pnumonia that consideres the main cause for depletation and  economic loses  of animals.