Prevalence of multidrug- resistant and blaVIM gene among carbapenem-resistant Klebsiella pneumoniae and Escherichia coli in children with throat infections in Hillah city

Abstract

The current work was designed to identify the occurrence of Klebsiella pneumoniae and Escherichia coli isolates and access the presence of bla_VIM gene among isolates with resistance to carbapenem. In total, 100 swabs were obtained from children with throat infections visited Babylon Teaching Hospital for Maternity and Pediatric, Hillah city, Babylon province between November, 2015 and February, 2016. Fourteen (77.78%) and 4 (22.22%) isolates were detected as K. pneumoniae and E. coli, respectively. The antimicrobial profile for all isolates was conducted using disk diffusion protocol. Both E. coli and K. pneumoniae exhibited higher level of resistant to penicillins antibiotics (ampicillin) with (75%) and (85.71%)  resistance rate, respectively. Resistant to penem agents was observed in two isolates of K. pneumoniae, which were further assayed  by PCR technique to detection the bla_VIM gene. One (50%) K. pneumoniae was positive for bla_VIM gene.