White mushroom Agaricus bisporus extract inhibits oxidative DNA damage in human lymphocytes as assessed by comet assay
DOI:
https://doi.org/10.63799/Keywords:
White mushroom,, Agaricus bisporus,, comet assay,, human lymphocyte,, DNA damage.Abstract
White mushroom Agaricus bisporus, one of the many known medicinal mushrooms, Agaricus bisporus
contains antioxidant activities. The antioxidant effects of the mushroom may be partly explained by
protection of cell components against free radicals. We evaluated the effect of aqueous Agaricus bisporus
extracts for their potential for protecting against oxidative damage to DNA in human lymphocytes. Cells
were pretreated with various concentra ons (10, 50, 100 and 500 μg/mL) of the extract (Sclerotium and
Mycelium) for 1 h at 37 oC. Cells were then treated with 100 μM of H2O2 for 5 min as an oxida ve stress.
Evaluation of oxidative damage was performed using single-cell gel electrophoresis for DNA
fragmentation (Comet assay). Using image analysis, the degree of DNA damage was evaluated as the DNA
tail moment. Cells pretreated with Agaricus bisporus extract showed over 30% reduc on in DNA
fragmenta on compared with the posi ve control (100 μmol H2O2 treatment). Thus, Agaricus bisporus
treatment affords cellular protection against endogenous DNA damage produced by H2O2.
