Studying the optimum conditions for cellulase production from wheat straw and papyrus straw using Trichoderma viride
DOI:
https://doi.org/10.63799/Keywords:
Trichoderma viride,, Solid state fermentation,, Cellulose,, Wheat straw,, Papyrus straw.Abstract
The cellulase production in solid state fermentation (SSF) by Trichoderma viride was investigated using
wheat straw and papyrus straw as the substrate. Present study described the optimization of process
parameters for the production of cellulases. The fermentation experiments were carried out in shake
flasks using pretreated wheat straw and papyrus straw. Maximum production of cellulases from wheat
straw (CMCase 1.62 U/ml/min) and from papyrus straw (PAPase 0.81 U/ml/min) was observed a er a
fermenta on period of 70hrs at an incuba on temperature of 30ºC. Ini al pH of the culture medium was
also op mized and a pH of 5.5 was found to support maximum growth and enzyme produc on. Different
inorganic nitrogen sources were evaluated for the production of cellulases and ammonium sulphate was
found to be the best. The enzyme production was further enhanced by carrying out fermentation
experiments using 25 ml of culture medium in 250 ml flask inoculated with 4% inoculum.
