Genetic characterization of OXA-1 β- lactamase producing carbapenem-resistant Klebsiella pneumoniae from Al-Hillah river water, Babylon Province, Iraq
DOI:
https://doi.org/10.63799/Keywords:
Klebsiella pneumoniae,, Carbapenem resistance,, OXA-1 beta lactamase,, PCR,, River water.Abstract
This study characterize the prevalence of Klebsiella pneumoniae harboring blaOXA-1 gene recovered from surface waters of Al-Hillah river. One hundred and one water samples were obtained from various environmental sites of Al-Hillah river between January and April 2015. Thirty five (34.6%) isolates were specified as K.pneumoniae. Their antimicrobial susceptibility profiles were investigated using disc diffusion method. The high level of resistance were detected for penicillin agents (ampicillin and piperacillin) with (57.14%) and (54.28%) resistance rate, respectively. Resistance to carbapenem was identified in 2 isolates of K.pneumoniae, these were further tested by Polymerase Chain Reaction (PCR) technique for the existence of blaOXA-1 gene,1 (50%) isolate with positive result.
